ارزیابی ارتباط ژنتیکی سالمونلا انتریکا سرووار انتریتیدیس جدا شده از نمونه ‏های انسانی و دامی به روش ‏ERIC- PCR

Background & Aims: Salmonellosis is one of the vital infectious zoonotic diseases in both human and animals that is related with poultry, meat, egg and milk consumption. The aim of this study was evaluation of genetic diversity of Salmonellaenterica serovar enteritidis isolated from human and animals samples by ERIC-PCR method. Materials & Methods: In this cross-sectional study, 60 Salmonellaenterica serovar enteritidis were obtained from the human and animals. Detection of strains were performed by standard microbiological and biochemical tests and slid agglutination assay were done for serotyping of the strains by mono and polyvalent antisera. Then, ERIC-PCR was achieved for determination of molecular correlation of the strains by specific oligonucleotides primers. Results: The results showed that, all 60 S. enterica using ERIC 1 and ERIC  were type II. And 2-11 bands with 20-3200 bp were obtained. Therefore, 15 different clusters (C1-C15) were attained and that highest number (13.3%, 8 strains) with like pattern were in cluster 5 (C5). Conclusion: The results showed that Salmonellaenterica serovar enteritidis strains are non-homolog. So, ERIC-PCR method is an appropriate method for molecular typing of Salmonella strains and infection spread source determination used for epidemiologic survey and infection prevention pathway. SOURCE: URMIA MED J 2016: 27(5): 418 ISSN: 1027-3727